This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027363
PrimePCR™ PreAmp for SYBR® Green Assay: FGF17, Human
PrimePCR™ Template for SYBR® Green Assay: FGF17, Human
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development cell growth morphogenesis tissue repair tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain the midbrain-hindbrain junction developing skeleton and developing arteries which suggests a role in central nervous system bone and vascular development. This gene was referred to as FGF-13 in reference 2 however its amino acid sequence and chromosomal localization are identical to FGF17. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.