PrimePCR™ SYBR® Green Assay: SLC6A17, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027338

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009823
Assay Design:   Intron-spanning
Chromosome Location:   1:110738300-110740157question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000331565 ENST00000450985

Gene Information

The SLC6 family of proteins which includes SLC6A17 acts as specific transporters for neurotransmitters amino acids and osmolytes like betaine taurine and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions (Hoglund et al. 2005 [PubMed 16125675]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC6A17
Gene Name:   solute carrier family 6, member 17
Aliases:   NTT4
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000197106
Entrez:   388662
UniGene:   Hs.128382
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.820000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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