PrimePCR™ SYBR® Green Assay: TRUB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027325

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009790
Assay Design:   Intron-spanning
Chromosome Location:   9:131073808-131076118question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000372890 ENST00000546104

Gene Information

Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al. 2003 [PubMed 12736709]).[supplied by OMIM Mar 2008]

Gene Symbol:   TRUB2
Gene Name:   TruB pseudouridine (psi) synthase homolog 2 (E. coli)
Aliases:   CLONE24922, RP11-339B21.1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000167112
Entrez:   26995
UniGene:   Hs.632685
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.780000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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