PrimePCR™ SYBR® Green Assay: PEX7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027317

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009768
Assay Design:   Intron-spanning
Chromosome Location:   6:137219315-137234700question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000318471 ENST00000541292

Gene Information

This gene encodes the cytosolic receptor for the set of peroxisomal matrix enzymes targeted to the organelle by the peroxisome targeting signal 2 (PTS2). Defects in this gene cause peroxisome biogenesis disorders (PBDs) which are characterized by multiple defects in peroxisome function. There are at least 14 complementation groups for PBDs with more than one phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene have been associated with PBD complementation group 11 (PBD-CG11) disorders rhizomelic chondrodysplasia punctata type 1 (RCDP1) and Refsum disease (RD). [provided by RefSeq Oct 2008]

Gene Symbol:   PEX7
Gene Name:   peroxisomal biogenesis factor 7
Aliases:   PTS2R, RCDP1, RD
RefSeq:   NC_000006.11 NG_008462.1 NT_025741.15
Ensembl:   ENSG00000112357
Entrez:   5191
UniGene:   Hs.280932
Chromosome Mapping:   6q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.280000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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