This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NKAIN2, Human
PrimePCR™ Template for SYBR® Green Assay: NKAIN2, Human
The protein encoded by this gene is a transmembrane protein that interacts with the beta subunit of NaK-ATPase (ATP1B1). A chromosomal translocation involving this gene is a cause of lymphoma. At least two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.