PrimePCR™ SYBR® Green Assay: CDH11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009695
Assay Design:   Intron-spanning
Chromosome Location:   16:65032716-65038613question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000268603 ENST00000394156 ENST00000536902

Gene Information

This gene encodes a type II classical cadherin from the cadherin superfamily integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain a single membrane-spanning domain and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines and its upregulation during differentiation suggests a specific function in bone development and maintenance. [provided by RefSeq Jul 2008]

Gene Symbol:   CDH11
Gene Name:   cadherin 11, type 2, OB-cadherin (osteoblast)
Aliases:   CAD11, CDHOB, OB, OSF-4
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140937
Entrez:   1009
UniGene:   Hs.116471
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.570000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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