PrimePCR™ SYBR® Green Assay: CNGA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009650
Assay Design:   Intron-spanning
Chromosome Location:   2:99008365-99012343question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000436404 ENST00000272602 ENST00000409937 ENST00000393504

Gene Information

This gene encodes a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in this gene are associated with achromatopsia (rod monochromacy) and color blindness. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   CNGA3
Gene Name:   cyclic nucleotide gated channel alpha 3
Aliases:   ACHM2, CCNC1, CCNCa, CCNCalpha, CNCG3, CNG3
RefSeq:   NC_000002.11 NT_022171.15 NG_009097.1
Ensembl:   ENSG00000144191
Entrez:   1261
UniGene:   Hs.234785
Chromosome Mapping:   2q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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