PrimePCR™ SYBR® Green Assay: SRRM4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009623
Assay Design:   Intron-spanning
Chromosome Location:   12:119552133-119558072question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000267260

Gene Information

SRRM4 promotes alternative splicing and inclusion of neural-specific exons in target mRNAs (Calarco et al. 2009 [PubMed 19737518]).[supplied by OMIM Oct 2009]

Gene Symbol:   SRRM4
Gene Name:   serine/arginine repetitive matrix 4
Aliases:   KIAA1853, MGC176729, MU-MB-2.76, nSR100
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000139767
Entrez:   84530
UniGene:   Hs.112577
Chromosome Mapping:   12q24.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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