This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027254
PrimePCR™ PreAmp for SYBR® Green Assay: SCN1A, Human
PrimePCR™ Template for SYBR® Green Assay: SCN1A, Human
The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials mainly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and two smaller auxiliary beta subunits. This gene encodes the large alpha subunit and mutations in this gene have been associated with several epilepsy convulsion and migraine disorders. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons the exact combination of which have not been experimentally confirmed for the full-length transcript.[provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.