PrimePCR™ SYBR® Green Assay: PLA2G7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009578
Assay Design:   Intron-spanning
Chromosome Location:   6:46682266-46684191question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000274793 ENST00000537365 ENST00000538237

Gene Information

The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Dec 2009]

Gene Symbol:   PLA2G7
Gene Name:   phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Aliases:   LDL-PLA2, LP-PLA2, PAFAH
RefSeq:   NC_000006.11 NG_016204.1 NT_007592.15
Ensembl:   ENSG00000146070
Entrez:   7941
UniGene:   Hs.584823
Chromosome Mapping:   6p21.2-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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