PrimePCR™ SYBR® Green Assay: BEGAIN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027247

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009576
Assay Design:   Intron-spanning
Chromosome Location:   14:101010199-101011396question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000355173 ENST00000556751 ENST00000553553 ENST00000556188 ENST00000554356 ENST00000557378 ENST00000554140 ENST00000443071

Gene Information

Description Not Available

Gene Symbol:   BEGAIN
Gene Name:   brain-enriched guanylate kinase-associated homolog (rat)
Aliases:   KIAA1446
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000183092
Entrez:   57596
UniGene:   Hs.211751
Chromosome Mapping:   14q32.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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