PrimePCR™ SYBR® Green Assay: TMX4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009534
Assay Design:   Intron-spanning
Chromosome Location:   20:7980451-7990877question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000246024

Gene Information

Description Not Available

Gene Symbol:   TMX4
Gene Name:   thioredoxin-related transmembrane protein 4
Aliases:   DJ971N18.2, KIAA1162, PDIA14, TXNDC13
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000125827
Entrez:   56255
UniGene:   Hs.169358
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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