PrimePCR™ SYBR® Green Assay: FZD6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027202

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FZD6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FZD6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009457
Assay Design:   Intron-spanning
Chromosome Location:   8:104330887-104336743question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000522566 ENST00000523739 ENST00000358755 ENST00000539487

Gene Information

This gene represents a member of the 'frizzled' gene family which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide a cysteine-rich domain in the N-terminal extracellular region and seven transmembrane domains but unlike other family members this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade thereby inhibiting the processes that trigger oncogenic transformation cell proliferation and inhibition of apoptosis. Alternative splicing results in multiple transcript variants some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq Aug 2011]

Gene Symbol:   FZD6
Gene Name:   frizzled homolog 6 (Drosophila)
Aliases:   FZ6, HFZ6
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000164930
Entrez:   8323
Chromosome Mapping:   8q22.3-q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download