PrimePCR™ SYBR® Green Assay: SGK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027199

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009449
Assay Design:   Intron-spanning
Chromosome Location:   20:42208637-42213558question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000373100 ENST00000373092 ENST00000373077 ENST00000341458 ENST00000426287 ENST00000423407

Gene Information

This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK) this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2010]

Gene Symbol:   SGK2
Gene Name:   serum/glucocorticoid regulated kinase 2
Aliases:   H-SGK2, dJ138B7.2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101049
Entrez:   10110
UniGene:   Hs.300863
Chromosome Mapping:   20q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.350000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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