This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC38A1, Human
PrimePCR™ Template for SYBR® Green Assay: SLC38A1, Human
Amino acid transporters play essential roles in the uptake of nutrients production of energy chemical metabolism detoxification and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine an intermediate in the detoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter glutamate (Gu et al. 2001 [PubMed 11325958]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.