PrimePCR™ SYBR® Green Assay: NMUR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009406
Assay Design:   Intron-spanning
Chromosome Location:   5:151775115-151783979question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000255262

Gene Information

This gene encodes a protein from the G-protein coupled receptor 1 family. This protein is a receptor for neuromedin U which is a neuropeptide that is widely distributed in the gut and central nervous system. This receptor plays an important role in the regulation of food intake and body weight. [provided by RefSeq Jul 2008]

Gene Symbol:   NMUR2
Gene Name:   neuromedin U receptor 2
Aliases:   FM-4, FM4, NMU-R2, NMU2R, TGR-1, TGR1
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000132911
Entrez:   56923
UniGene:   Hs.731911
Chromosome Mapping:   5q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.720000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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