PrimePCR™ SYBR® Green Assay: FAM186B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009404
Assay Design:   Intron-spanning
Chromosome Location:   12:49994814-49997007question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000551047 ENST00000257894 ENST00000544141

Gene Information

This gene product is a member of the FAM186 family however its exact function is not known. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   FAM186B
Gene Name:   family with sequence similarity 186, member B
Aliases:   C12orf25, DKFZp434J0113, DKFZp434N2350
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135436
Entrez:   84070
UniGene:   Hs.524406
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.150000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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