PrimePCR™ SYBR® Green Assay: GRHL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009368
Assay Design:   Intron-spanning
Chromosome Location:   8:102649145-102661687question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000251808 ENST00000395927 ENST00000395928

Gene Information

The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).[provided by RefSeq Mar 2009]

Gene Symbol:   GRHL2
Gene Name:   grainyhead-like 2 (Drosophila)
Aliases:   BOM, DFNA28, FLJ11172, FLJ13782, MGC149294, MGC149295, TFCP2L3
RefSeq:   NC_000008.10 NG_011971.1 NT_008046.16
Ensembl:   ENSG00000083307
Entrez:   79977
UniGene:   Hs.661088
Chromosome Mapping:   8q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.490000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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