PrimePCR™ SYBR® Green Assay: BPNT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027146

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009321
Assay Design:   Intron-spanning
Chromosome Location:   1:220247340-220253121question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000354807 ENST00000463953 ENST00000498791 ENST00000498237 ENST00000302686 ENST00000322067 ENST00000414869 ENST00000469520

Gene Information

BPNT1 also called bisphosphate 3-prime-nucleotidase or BPntase is a member of a magnesium-dependent phosphomonoesterase family. Lithium a major drug used to treat manic depression acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq Jul 2008]

Gene Symbol:   BPNT1
Gene Name:   3'(2'), 5'-bisphosphate nucleotidase 1
Aliases:   PIP
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162813
Entrez:   10380
UniGene:   Hs.406134
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.260000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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