PrimePCR™ SYBR® Green Assay: FYTTD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027134

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009292
Assay Design:   Intron-spanning
Chromosome Location:   3:197501021-197503868question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000428738 ENST00000415708 ENST00000241502 ENST00000424384 ENST00000428395

Gene Information

Description Not Available

Gene Symbol:   FYTTD1
Gene Name:   forty-two-three domain containing 1
Aliases:   DKFZp761B1514, UIF
RefSeq:   NC_000003.11 NT_029928.13
Ensembl:   ENSG00000122068
Entrez:   84248
UniGene:   Hs.277533
Chromosome Mapping:   3q29

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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