PrimePCR™ SYBR® Green Assay: ATG16L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009284
Assay Design:   Intron-spanning
Chromosome Location:   2:234198918-234200811question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000392017 ENST00000373525 ENST00000392020 ENST00000392018 ENST00000347464 ENST00000334050

Gene Information

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jun 2010]

Gene Symbol:   ATG16L1
Gene Name:   ATG16 autophagy related 16-like 1 (S. cerevisiae)
Aliases:   APG16L, ATG16A, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
RefSeq:   NC_000002.11 NT_005120.16 NG_023038.1
Ensembl:   ENSG00000085978
Entrez:   55054
UniGene:   Hs.529322
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 35.280000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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