PrimePCR™ SYBR® Green Assay: RAB11FIP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027122

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009271
Assay Design:   Intron-spanning
Chromosome Location:   16:560680-568983question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000262305 ENST00000434585 ENST00000457159 ENST00000450428 ENST00000448401

Gene Information

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation targeting and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al. 2001 [PubMed 11495908]).[supplied by OMIM Mar 2008]

Gene Symbol:   RAB11FIP3
Gene Name:   RAB11 family interacting protein 3 (class II)
Aliases:   KIAA0665, Rab11-FIP3
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000090565
Entrez:   9727
UniGene:   Hs.531642
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.890000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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