PrimePCR™ SYBR® Green Assay: ZC3H11A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027112

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009252
Assay Design:   Intron-spanning
Chromosome Location:   1:203817466-203818910question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000367214 ENST00000367212 ENST00000332127 ENST00000367210 ENST00000545588 ENST00000537080

Gene Information

Description Not Available

Gene Symbol:   ZC3H11A
Gene Name:   zinc finger CCCH-type containing 11A
Aliases:   DKFZp686D03108, DKFZp686F14109, DKFZp781G2455, KIAA0663, ZC3HDC11A
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000058673
Entrez:   9877
UniGene:   Hs.532399
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.420000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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