PrimePCR™ SYBR® Green Assay: KDM6A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027106

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KDM6A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KDM6A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009242
Assay Design:   Intron-spanning
Chromosome Location:   X:44922967-44928862question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000377967 ENST00000414389 ENST00000433797 ENST00000536777 ENST00000382899 ENST00000543216 ENST00000542299 ENST00000334516 ENST00000535688

Gene Information

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq Nov 2009]

Gene Symbol:   KDM6A
Gene Name:   lysine (K)-specific demethylase 6A
Aliases:   DKFZp686A03225, MGC141941, UTX, bA386N14.2
RefSeq:   NC_000023.10 NG_016260.1 NT_079573.4
Ensembl:   ENSG00000147050
Entrez:   7403
Chromosome Mapping:   Xp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.730000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download