PrimePCR™ SYBR® Green Assay: AGPS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027097

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009220
Assay Design:   Intron-spanning
Chromosome Location:   2:178333217-178357883question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000536686 ENST00000264167

Gene Information

This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata type 3 and Zellweger syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   AGPS
Gene Name:   alkylglycerone phosphate synthase
Aliases:   ADAP-S, ADAS, ADHAPS, ADPS, ALDHPSY, DKFZp762O2215, FLJ99755
RefSeq:   NC_000002.11 NG_008968.1 NT_005403.17
Ensembl:   ENSG00000018510
Entrez:   8540
UniGene:   Hs.516543
Chromosome Mapping:   2q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.870000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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