PrimePCR™ SYBR® Green Assay: ZEB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009210
Assay Design:   Intron-spanning
Chromosome Location:   10:31810722-31812896question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000320985 ENST00000437844 ENST00000537225 ENST00000361642 ENST00000546250 ENST00000542815 ENST00000446923 ENST00000543514 ENST00000542879

Gene Information

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq Mar 2010]

Gene Symbol:   ZEB1
Gene Name:   zinc finger E-box binding homeobox 1
Aliases:   AREB6, BZP, DELTAEF1, FECD6, MGC133261, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
RefSeq:   NC_000010.10 NT_008705.16 NG_017048.1
Ensembl:   ENSG00000148516
Entrez:   6935
UniGene:   Hs.124503
Chromosome Mapping:   10p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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