PrimePCR™ SYBR® Green Assay: PLEKHA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009189
Assay Design:   Intron-spanning
Chromosome Location:   8:38810832-38814893question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000420274 ENST00000535929

Gene Information

Description Not Available

Gene Symbol:   PLEKHA2
Gene Name:   pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
Aliases:   FLJ25921, TAPP2
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000169499
Entrez:   59339
UniGene:   Hs.369123
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.630000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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