PrimePCR™ SYBR® Green Assay: KIF1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027081

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIF1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIF1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009182
Assay Design:   Intron-spanning
Chromosome Location:   1:10336379-10338121question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000263934 ENST00000377093 ENST00000377086 ENST00000377083 ENST00000377081 ENST00000355249

Gene Information

This gene encodes a motor protein that transports mitochondria and synaptic vesicle precursors. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A1. [provided by RefSeq Jul 2008]

Gene Symbol:   KIF1B
Gene Name:   kinesin family member 1B
Aliases:   CMT2, CMT2A, CMT2A1, FLJ23699, HMSNII, KIAA0591, KIAA1448, KLP, MGC134844, NBLST1
RefSeq:   NC_000001.10 NG_008069.1 NT_021937.19
Ensembl:   ENSG00000054523
Entrez:   23095
Chromosome Mapping:   1p36.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.570000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download