PrimePCR™ SYBR® Green Assay: DOCK4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027069

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DOCK4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DOCK4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009157
Assay Design:   Intron-spanning
Chromosome Location:   7:111405262-111409640question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000423057 ENST00000428084 ENST00000437633 ENST00000445943 ENST00000437129 ENST00000450156 ENST00000352877 ENST00000342288 ENST00000494651

Gene Information

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian prostate glioma and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described but only one has been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   DOCK4
Gene Name:   dedicator of cytokinesis 4
Aliases:   FLJ34238, KIAA0716, MGC134911, MGC134912
RefSeq:   NC_000007.13 NG_028060.1 NT_007933.15
Ensembl:   ENSG00000128512
Entrez:   9732
Chromosome Mapping:   7q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download