PrimePCR™ SYBR® Green Assay: ENOX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ENOX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ENOX1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008889
Assay Design:   Intron-spanning
Chromosome Location:   13:43896525-43900599question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000261488 ENST00000412891 ENST00000540032

Gene Information

Electron transport pathways are generally associated with mitochondrial membranes but non-mitochondrial pathways are also biologically significant. Plasma membrane electron transport pathways are involved in functions as diverse as cellular defense intracellular redox homeostasis and control of cell growth and survival. Members of the ecto-NOX family such as CNOX or ENOX1 are involved in plasma membrane transport pathways. These enzymes exhibit both a hydroquinone (NADH) oxidase activity and a protein disulfide-thiol interchange activity in series with each activity cycling every 22 to 26 minutes (Scarlett et al. 2005 [PubMed 15882838]).[supplied by OMIM Mar 2008]

Gene Symbol:   ENOX1
Gene Name:   ecto-NOX disulfide-thiol exchanger 1
Aliases:   CNOX, FLJ10094, PIG38, bA64J21.1, cCNOX
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000120658
Entrez:   55068
Chromosome Mapping:   13q14.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.780000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download