PrimePCR™ SYBR® Green Assay: HAT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HAT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008873
Assay Design:   Intron-spanning
Chromosome Location:   2:172841189-172844228question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000264108 ENST00000392584

Gene Information

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation particularly of histone H4 plays an important role in replication-dependent chromatin assembly. Specifically this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12 and to a lesser degree histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Jun 2009]

Gene Symbol:   HAT1
Gene Name:   histone acetyltransferase 1
Aliases:   KAT1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000128708
Entrez:   8520
Chromosome Mapping:   2q31.2-q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.650000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download