This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ZNF75D, Human
PrimePCR™ Template for SYBR® Green Assay: ZNF75D, Human
This gene encodes a protein that likely functions as a transcription factor. The protein which belongs to the ZNF75 family includes an N-terminal SCAN domain a KRAB box and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16 while pseudogenes have been identified on chromosomes 11 and 12. Alternative splicing results in multiple transcripts variants. [provided by RefSeq Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.