PrimePCR™ SYBR® Green Assay: SH3D19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026948

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SH3D19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SH3D19, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008785
Assay Design:   Intron-spanning
Chromosome Location:   4:152060927-152065120question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000409598 ENST00000409252 ENST00000514152 ENST00000304527 ENST00000455740 ENST00000424281 ENST00000427414

Gene Information

This gene encodes a multiple SH3 domain-containing protein which interacts with other proteins such as EBP and members of ADAM family via the SH3 domains. This protein may be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1 by EBP and regulation of ADAM proteins in the signaling of EGFR-ligand shedding. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   SH3D19
Gene Name:   SH3 domain containing 19
Aliases:   EBP, EVE1, Kryn, MGC105136, MGC118910, MGC118911, MGC118912, MGC118913, SH3P19
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000109686
Entrez:   152503
Chromosome Mapping:   4q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.880000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download