This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026896
PrimePCR™ PreAmp for SYBR® Green Assay: KCNIP3, Human
PrimePCR™ Template for SYBR® Green Assay: KCNIP3, Human
This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins which belong to the recoverin branch of the EF-hand superfamily. Members of this family are small calcium binding proteins containing EF-hand-like domains. They are integral subunit components of native Kv4 channel complexes that may regulate A-type currents and hence neuronal excitability in response to changes in intracellular calcium. The encoded protein also functions as a calcium-regulated transcriptional repressor and interacts with presenilins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.