PrimePCR™ SYBR® Green Assay: ZNF182, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026666

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF182, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF182, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008156
Assay Design:   Intron-spanning
Chromosome Location:   X:47837142-47842686question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000376943 ENST00000396965 ENST00000305127

Gene Information

Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions including cell proliferation differentiation and apoptosis. This gene encodes a zinc finger protein and belongs to the krueppel C2H2-type zinc-finger protein family. It may be involved in transcriptional regulation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq May 2010]

Gene Symbol:   ZNF182
Gene Name:   zinc finger protein 182
Aliases:   HHZ150, KOX14, MGC125383, MGC131713, ZNF21, Zfp182
RefSeq:   NC_000023.10 NG_021254.1 NT_079573.4
Ensembl:   ENSG00000147118
Entrez:   7569
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.970000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download