This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026442
PrimePCR™ PreAmp for SYBR® Green Assay: MTMR7, Human
PrimePCR™ Template for SYBR® Green Assay: MTMR7, Human
This gene encodes a member of the myotubularin family of tyrosine/dual-specificity phosphatases. The encoded protein is characterized by four distinct domains that are conserved among all members of the myotubularin family: the glucosyltransferase Rab-like GTPase activator and myotubularins domain the Rac-induced recruitment domain the protein tyrosine phosphatases and dual-specificity phosphatases domain and the suppressor of variegation 3-9 enhancer-of-zeste and trithorax interaction domain. This protein dephosphorylates the target substrates phosphatidylinositol 3-phosphate and inositol 13-bisphosphate. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq Mar 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.