PrimePCR™ SYBR® Green Assay: ADAMTS9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ADAMTS9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADAMTS9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007331
Assay Design:   Intron-spanning
Chromosome Location:   3:64636793-64640104question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000295903 ENST00000498707 ENST00000459780

Gene Information

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules including a propeptide region a metalloproteinase domain a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans the control of organ shape during development and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2 an area known to be lost in hereditary renal tumors. [provided by RefSeq Jul 2008]

Gene Symbol:   ADAMTS9
Gene Name:   ADAM metallopeptidase with thrombospondin type 1 motif, 9
Aliases:   FLJ42955, KIAA1312
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163638
Entrez:   56999
Chromosome Mapping:   3p14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.520000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download