PrimePCR™ SYBR® Green Assay: LAMA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LAMA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LAMA2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007207
Assay Design:   Intron-spanning
Chromosome Location:   6:129775392-129777607question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000421865 ENST00000358023 ENST00000354729 ENST00000443169

Gene Information

Laminin an extracellular protein is a major component of the basement membrane. It is thought to mediate the attachment migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits alpha beta and gamma which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LAMA2
Gene Name:   laminin, alpha 2
Aliases:   LAMM
RefSeq:   NC_000006.11 NG_008678.1 NT_025741.15
Ensembl:   ENSG00000196569
Entrez:   3908
Chromosome Mapping:   6q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.520000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download