PrimePCR™ SYBR® Green Assay: GRHL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026379

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GRHL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GRHL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007198
Assay Design:   Intron-spanning
Chromosome Location:   1:24658058-24663023question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000361548 ENST00000350501 ENST00000356046 ENST00000524724 ENST00000236255 ENST00000342072

Gene Information

This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq Aug 2010]

Gene Symbol:   GRHL3
Gene Name:   grainyhead-like 3 (Drosophila)
Aliases:   MGC46624, SOM, TFCP2L4
RefSeq:   NC_000001.10 NT_004610.19 NG_009308.1
Ensembl:   ENSG00000158055
Entrez:   57822
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.100000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download