This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026347
PrimePCR™ PreAmp for SYBR® Green Assay: MPP6, Human
PrimePCR™ Template for SYBR® Green Assay: MPP6, Human
Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane cytoskeletal and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies DLG-like (see DLG1; MIM 601014) ZO1-like (see TJP1; MIM 601009) p55-like (see MPP1; MIM 305360) and LIN2-like (see CASK; MIM 300172) based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily (Tseng et al. 2001 [PubMed 11311936]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.