PrimePCR™ SYBR® Green Assay: SEMA6A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SEMA6A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEMA6A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007056
Assay Design:   Intron-spanning
Chromosome Location:   5:115833029-115837980question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000343348 ENST00000257414 ENST00000510263 ENST00000515009 ENST00000509665

Gene Information

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al. 2001 [PubMed 11242070]).[supplied by OMIM Feb 2011]

Gene Symbol:   SEMA6A
Gene Name:   sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Aliases:   HT018, KIAA1368, SEMA, SEMA6A1, SEMAQ, VIA
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000092421
Entrez:   57556
Chromosome Mapping:   5q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.610000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download