This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: GLRB, Human
PrimePCR™ Template for SYBR® Green Assay: GLRB, Human
This gene encodes the beta subunit of the glycine receptor which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease also known as hereditary hyperekplexia or congenital stiff-person syndrome a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.