This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NSD1, Human
PrimePCR™ Template for SYBR® Green Assay: NSD1, Human
This gene encodes a protein containing a SET domain 2 LXXLL motifs 3 nuclear translocation signals (NLSs) 4 plant homeodomain (PHD) finger regions and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var enhancer of zeste and trithorax domain protein 1 on chromosome 5 and nucleoporin 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.