PrimePCR™ SYBR® Green Assay: ACRBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ACRBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACRBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006707
Assay Design:   Intron-spanning
Chromosome Location:   12:6754440-6755686question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000229243 ENST00000414226 ENST00000536350 ENST00000546114

Gene Information

The protein encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse guinea pig and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues this mRNA is expressed only in testis whereas it is detected in a range of different tumor types such as bladder breast lung liver and colon. [provided by RefSeq Jul 2008]

Gene Symbol:   ACRBP
Gene Name:   acrosin binding protein
Aliases:   CT23, FLJ51160, OY-TES-1, SP32
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000111644
Entrez:   84519
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 34.440000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download