PrimePCR™ SYBR® Green Assay: GRIPAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026207

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GRIPAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GRIPAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006536
Assay Design:   Intron-spanning
Chromosome Location:   X:48834793-48837673question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000376425 ENST00000376444 ENST00000376441 ENST00000376423 ENST00000537291

Gene Information

This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). In brain studies the encoded protein was found with the GRIP/AMPA receptor complex. Multiple alternatively spliced transcript variants have been described that encode different protein isoforms; however the full-length nature and biological validity of all of these variants have not been determined.[provided by RefSeq Nov 2009]

Gene Symbol:   GRIPAP1
Gene Name:   GRIP1 associated protein 1
Aliases:   DKFZp434P0630, GRASP-1, KIAA1167, MGC126593, MGC126595
RefSeq:   NC_000023.10 NG_012515.1 NG_016263.1 NT_079573.4
Ensembl:   ENSG00000068400
Entrez:   56850
Chromosome Mapping:   Xp11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.530000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download