PrimePCR™ SYBR® Green Assay: SRCAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026195

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SRCAP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SRCAP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006510
Assay Design:   Intron-spanning
Chromosome Location:   16:30740838-30744704question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000262518 ENST00000395059 ENST00000344771

Gene Information

This gene encodes the core catalytic component of the multiprotein chromatin-remodeling SRCAP complex. The encoded protein is an ATPase that is necessary for the incorporation of the histone variant H2A.Z into nucleosomes. It can function as a transcriptional activator in Notch-mediated CREB-mediated and steroid receptor-mediated transcription. Mutations in this gene cause Floating-Harbor syndrome a rare disorder characterized by short stature language deficits and dysmorphic facial features. [provided by RefSeq Feb 2012]

Gene Symbol:   SRCAP
Gene Name:   Snf2-related CREBBP activator protein
Aliases:   DOMO1, EAF1, FLJ44499, KIAA0309, SWR1
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000080603
Entrez:   10847
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.210000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download