This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026187
PrimePCR™ PreAmp for SYBR® Green Assay: LAMB1, Human
PrimePCR™ Template for SYBR® Green Assay: LAMB1, Human
Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha beta and gamma (formerly A B1 and B2 respectively) and they form a cruciform structure consisting of 3 short arms each formed by a different chain and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown but some of the chains have been shown to differ with respect to their tissue distribution presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha domains III and V contain several EGF-like repeats and domains IV and VI have a globular conformation. Laminin beta 1 is expressed in most tissues that produce basement membranes and is one of the 3 chains constituting laminin 1 the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment chemotaxis and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.