PrimePCR™ SYBR® Green Assay: ART4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026148

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ART4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ART4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006412
Assay Design:   Intron-spanning
Chromosome Location:   12:14994027-14996002question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000420600 ENST00000430129 ENST00000228936

Gene Information

This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants some of which lead to adverse transfusion reactions are known. [provided by RefSeq Jul 2008]

Gene Symbol:   ART4
Gene Name:   ADP-ribosyltransferase 4 (Dombrock blood group)
Aliases:   CD297, DO, DOK1
RefSeq:   NC_000012.11 NG_007477.1 NT_009714.17
Ensembl:   ENSG00000111339
Entrez:   420
Chromosome Mapping:   12p13-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.730000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download