This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: LNPEP, Human
PrimePCR™ Template for SYBR® Green Assay: LNPEP, Human
This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin oxytocin lys-bradykinin met-enkephalin dynorphin A and other peptide hormones. The protein can be secreted in maternal serum reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4 or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.