PrimePCR™ Probe Assay: GIPC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best negative control

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GIPC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GIPC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058583
Assay Design:   exonic
Chromosome Location:   19:14591118-14591252question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000393033 ENST00000345425 ENST00000586027 ENST00000591349 ENST00000587210 ENST00000587969 ENST00000393029 ENST00000393028 ENST00000360319 ENST00000369850 ENST00000344736 ENST00000422373 ENST00000596447 ENST00000597475 ENST00000600520 ENST00000599487

Gene Information

GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al. 2008 [PubMed 18775991]).[supplied by OMIM Apr 2009]

Gene Symbol:   GIPC1
Gene Name:   GIPC PDZ domain containing family, member 1
Aliases:   C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, NIP, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, TIP-2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000123159
Entrez:   10755
Chromosome Mapping:   19p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.390000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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